Cadmium Toxicity Induced Changes in Nitrogen Management in Lycopersicon esculentum Leading to a Metabolic Safeguard Through an Amino Acid Storage Strategy
Identifieur interne : 000188 ( Main/Exploration ); précédent : 000187; suivant : 000189Cadmium Toxicity Induced Changes in Nitrogen Management in Lycopersicon esculentum Leading to a Metabolic Safeguard Through an Amino Acid Storage Strategy
Auteurs : Chiraz Chaffei [France, Tunisie] ; Karine Pageau [France] ; Akira Suzuki [France] ; Houda Gouia [Tunisie] ; Mohamed Habib Ghorbel [Tunisie] ; Céline Masclaux-Daubresse [France]Source :
- Plant and Cell Physiology [ 0032-0781 ] ; 2004-11-15.
English descriptors
- KwdEn :
- Abbreviations: Cd, cadmium; Fd-GOGAT, ferredoxin-dependent glutamate synthase; GaBa, γ-amino-n-butyric acid; GDH, glutamate dehydrogenase; GS, glutamine sunthetase; GS1, cytosolic GS; GS2, chloroplastic GS; ICDH, isocitrate dehydrogenase; MDH, malate dehydrogenase; NADH-GOGAT, NADH-dependent GOGAT; NAD+-MDH, NAD+-dependent MDH; NADH-MDH, NADH-dependent MDH; NADP+-MDH, NADP+-dependent MDH; NADPH-MDH, NADPH-dependent MDH; NR, nitrate reductase; NiR, nitrite reductase., Keywords: Cadmium stress — Lycopersicon esculentum — Nitrogen — Senescence..
Abstract
Tomato (Lycopersicon esculentum) seedlings were grown in the presence of cadmium. After 1 week of Cd treatment, a sharp decline in biomass accumulation in the leaves and roots was observed, together with a decrease in the rate of photosynthetic activity due to both Rubisco and chlorophyll degradation and stomata closure. Cadmium induced a significant decrease in nitrate content and inhibition of the activities of nitrate reductase, nitrite reductase, glutamine synthetase (GS) and ferredoxin-glutamate synthase. An increase in NADH-glutamate synthase and NADH-glutamate dehydrogenase activity was observed in parallel. The accumulation of ammonium into the tissues of treated plants was accompanied by a loss of total protein and the accumulation of amino acids. Gln represented the major amino acid transported through xylem sap of Cd-treated and control plants. Cadmium treatment increased the total amino acid content in the phloem, maintaining Gln/Glu ratios. Western and Northern blot analysis of Cd-treated plants showed a decrease in chloroplastic GS protein and mRNA and an increase in cytosolic GS and glutamate dehydrogenase transcripts and proteins. An increase in asparagine synthetase mRNA was observed in roots, in parallel with a strong increase in asparagine. Taken together, these results suggest that the plant response to Cd stress involved newly induced enzymes dedicated to coordinated leaf nitrogen remobilization and root nitrogen storage.
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DOI: 10.1093/pcp/pch192
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esculentum) seedlings were grown in the presence of cadmium. After 1 week of Cd treatment, a sharp decline in biomass accumulation in the leaves and roots was observed, together with a decrease in the rate of photosynthetic activity due to both Rubisco and chlorophyll degradation and stomata closure. Cadmium induced a significant decrease in nitrate content and inhibition of the activities of nitrate reductase, nitrite reductase, glutamine synthetase (GS) and ferredoxin-glutamate synthase. An increase in NADH-glutamate synthase and NADH-glutamate dehydrogenase activity was observed in parallel. The accumulation of ammonium into the tissues of treated plants was accompanied by a loss of total protein and the accumulation of amino acids. Gln represented the major amino acid transported through xylem sap of Cd-treated and control plants. Cadmium treatment increased the total amino acid content in the phloem, maintaining Gln/Glu ratios. Western and Northern blot analysis of Cd-treated plants showed a decrease in chloroplastic GS protein and mRNA and an increase in cytosolic GS and glutamate dehydrogenase transcripts and proteins. An increase in asparagine synthetase mRNA was observed in roots, in parallel with a strong increase in asparagine. Taken together, these results suggest that the plant response to Cd stress involved newly induced enzymes dedicated to coordinated leaf nitrogen remobilization and root nitrogen storage.</div></front></TEI><affiliations><list><country><li>France</li><li>Tunisie</li></country><region><li>Gouvernorat de Tunis</li><li>Île-de-France</li></region><settlement><li>Tunis</li><li>Versailles</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Chaffei, Chiraz" sort="Chaffei, Chiraz" uniqKey="Chaffei C" first="Chiraz" last="Chaffei">Chiraz Chaffei</name></region><name sortKey="Masclaux Daubresse, Ce Line" sort="Masclaux Daubresse, Ce Line" uniqKey="Masclaux Daubresse C" first="Céline" last="Masclaux-Daubresse">Céline Masclaux-Daubresse</name><name sortKey="Pageau, Karine" sort="Pageau, Karine" uniqKey="Pageau K" first="Karine" last="Pageau">Karine Pageau</name><name sortKey="Suzuki, Akira" sort="Suzuki, Akira" uniqKey="Suzuki A" first="Akira" last="Suzuki">Akira Suzuki</name></country><country name="Tunisie"><region name="Gouvernorat de Tunis"><name sortKey="Chaffei, Chiraz" sort="Chaffei, Chiraz" uniqKey="Chaffei C" first="Chiraz" last="Chaffei">Chiraz Chaffei</name></region><name sortKey="Ghorbel, Mohamed Habib" sort="Ghorbel, Mohamed Habib" uniqKey="Ghorbel M" first="Mohamed Habib" last="Ghorbel">Mohamed Habib Ghorbel</name><name sortKey="Gouia, Houda" sort="Gouia, Houda" uniqKey="Gouia H" first="Houda" last="Gouia">Houda Gouia</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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